needle puller p-97 (Sutter Instrument Company)

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Sutter Instrument Company needle puller p-97
Needle Puller P 97, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/needle+puller+p-97/micropipette+puller+p+97/pm40578366-290-24-27
Average 90 stars, based on 1 article reviews

needle puller p-97 - by Bioz Stars, 2026-09

90/100 stars

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Transplantation Assay:

Article Title: A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations.
Article Snippet: For xenotransplantation, a micromanipulator (Cat# M3301R, World Precision Instruments) holding a borosilicate glass capillary (Cat# GB100T-8P, without filament, Science Products) connected to a microinjector (FemtoJet 4i, Eppendorf) was used. .. Transplantation capillaries were pulled with a needle puller (P-97, Sutter Instruments) and loaded with approximately 5 μl of tumour cell suspension. ..

Suspension:

Injection:

Article Title: Refined high-content imaging-based phenotypic drug screening in zebrafish xenografts
Article Snippet: .. The borosilicate glass capillaries without filament (GB100T-8P, Science Products GmbH, Germany) for injection of tumor cells were pulled with a needle puller (P-97, Sutter Instruments, USA). .. Needles were loaded with ~5 μl of cell suspension, mounted onto a micromanipulator (M3301R, World Precision Instruments Inc., Germany) and connected to a microinjector (FemtoJet 4i, Eppendorf, Germany).

Article Title: High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-X L inhibition against Ewing sarcoma.
Article Snippet: .. For injection of tumor cells, borosilicate glass capillaries (GB100T-8 P, without filament, Science Products GmbH, Germany) pulled with a needle puller (P-97, Sutter Instruments, USA) were used. .. Needles were loaded with ~5 μl of tumor cell suspension, mounted onto a micromanipulator (M3301R, World Precision Instruments Inc., Germany) and S. Grissenberger et al. Cancer Letters 554 (2023) 216028 connected to a microinjector (FemtoJet 4i, Eppendorf, Germany).

Article Title: Left-right symmetry of zebrafish embryos requires somite surface tension.
Article Snippet: The body axis of vertebrate embryos is periodically segmented into bilaterally symmetric pairs of somites.. The anteroposterior length of somites, their position and left–right symmetry are thought to be molecularly determined before somite morphogenesis.. Here we show that, in zebrafish embryos, initial somite anteroposterior lengths and positions are imprecise and, consequently, many somite pairs form left–right asymmetrically.

Article Title: First dual inhibitors of human topoisomerase IIα and Hsp90 C-terminal domain inhibit the growth of Ewing sarcoma in vitro and in vivo.
Article Snippet: .. For tumor cell injection, borosilicate glass capillaries (GB100T-8P, without filament, Science Products GmbH, Germany) were pulled with a needle puller (P-97, Sutter Instruments, USA). ..

Microinjection:

Article Title: zHORSE as an optogenetic zebrafish strain for precise spatiotemporal control over gene expression during development.
Article Snippet: ‘‘loxP-EWS::FLI1’’ plasmid pTol2-ubi:loxPBFPloxP-mScarlet-P2A-EWS::FLI1 and ‘‘loxP-NRASG12D’’ plasmid pTol2-ubi:loxPBFPloxP-mScarlet-P2ANRASG12D were constructed via Gibson assembly using NEBuilder HiFi DNA Assembly kit (New England Biolabs). mRNA synthesis Synthesis of GAVPO, iCreV and Tol2 mRNA was performed using a mMessage mMachine SP6 transcription kit (Thermo Fisher) after linearization of respective plasmids with NotI (New England Biolabs). .. For microinjection of plasmid DNA and mRNA, borosilicate glass capillaries with filament (GB100F-10, Science Products GmbH, Hofheim am Taunus, Germany) were pulled with a needle puller (P-97, Sutter Instruments, Novato, CA, USA). .. Needles were loaded with 3 μl of DNA/mRNA injection mix (nuclease-free water, phenol red solution, DNA/mRNA), mounted onto a micromanipulator (M3301R, World Precision Instruments Inc., Sarasota, FL, USA) and connected to a microinjector (FemtoJet 4i, Eppendorf, Germany).

Plasmid Preparation:

other:

Article Title: Diagnostic Accuracy of Cerebrospinal Fluid Presepsin as a Marker of Bacterial Meningitis in Patients with External Ventricular Drainage – A Cross-Sectional Study in a Tertiary Intensive Care Unit
Article Snippet: For mRNA transcription, SP6 mMessage mMachine Kit (Ambion) was used and mRNAs were injected via glass capillaries (30-0020, Harvard Apparatus, pulled by a needle puller P-97, Sutter Instruments) while mounted on a microinjection system (PV820, World Precision Instruments).

Article Title: Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
Article Snippet: Needle puller , Sutter Instrument , P-97 , .

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